Day 1
Introduction, instrumentation & Praticals
Day 2
Instrumentation practicals II + quality control
Day 3
Processing and analysing bioimage data
9:00 AM
— 9:30 AM
Welcome & intro to the workshop
9:30 AM
— 11:00 AM
Deconstructing a microscope and basic care
- The compound microscope
- Disassembly and reassembly of a compound microscope
- Light paths & image formation
- Essential care of an optical microscope and components
COFFEE BREAK
11:45 AM
— 12:15 PM
Illumination and filters in light microscopy
- Bright-field illumination techniques
- Lasers and LEDs as sources for fluorescence illumination
- Fluorescence filters, spectral filtering and assessing illumination quality
12:45 PM
Sponsor pitch
LUNCH
1:45 PM
— 2:15 PM
Sponsors talks
2:15 PM
— 3:00 PM
Image detectors & Digital images
- Cameras vs point-detectors. Quantum efficiency and dynamic range. Signal vs backgroung vs noise (& types of noise).
- Detectors characteristics and limitations.
- Bioimage digital formats & managing bioimage data.
3:30 PM
— 4:00 PM
Optical sectioning methods - inside the box & choosing the right tool
Topics addressed:
- CLSM
- SDCM
- 2P
- Light-sheet
- OPT
COFFEE BREAK
4:15 PM
Sponsors pitch
8:00 PM
— 10:00 PM
Dinner (@ "A Quinta" near train station)
9:00 AM
— 11:30 AM
Optimizing CSLM acquisition + Airyscan
COFFEE BREAK
11:15 AM
— 12:30 PM
Non-diffraction-limited imaging & nanoscopy techniques (Round Table)
Some of the topics addressed:
- TIRF, ISM, SIM, STORM etc...
12:00 PM
Advanced fluorescence techniques: F techniques & photomanipulation (Round Table)
Some of the topics addressed:
- FRAP, FLIP
- Photoablation
- FRET
Sponsor pitch
LUNCH
Sponsor pitch
2:00 PM
— 3:00 PM
Special sample preparation (tissue clearing & expansion microscopy)
Do's and don'ts of tissue clearing and expansion microscopy.
Tested protocols, and how to image cleared and expanded samples.
3:00 PM
— 4:00 PM
Basic maintenance and QC in microscopy instrumentation
COFFEE BREAK
Sponsor pitch
Wine & cheese reception (at the canteen)
free night (perhaps drinks at the BeerHunter?
9:00 AM
— 11:00 AM
The "right" BiAs tools for each project
Round table with experts in:
FIJI
QuPath
CellProfiler
Imaris
Python and AI
COFFEE BREAK
11:15 AM
— 12:00 PM
Deconvolution with PPBI Huygens
12:00 PM
— 1:00 PM
Imaris in PPBI - Beyound the basics
LUNCH
2:00 PM
— 5:00 PM
Train the trainer & management (Round Table)
COFFEE BREAK
Conclusion...